Research Integrity and Assurance

Training Requirements

Animals: training information

To use any live vertebrate animals at ASU, all faculty, staff, graduate students and undergraduate researchers must:

  1. achieve certification in the animal care and use training program
  2. receive clearance from the Occupational Health and Safety Program
  3. The only individuals allowed to work with animals without meeting these requirements are students enrolled in a formal ASU course (one that has a university course number, such as BIO361) that includes the use of live animals. In such courses, the students are directly supervised by the instructor or teaching assistant, both of whom must be listed on an IACUC protocol. Individuals receiving live animal training from researchers proficient in the techniques or from DACT personnel must be on an ASU IACUC approved protocol before receiving that training.

    The ASU animal training is comprised of three levels:

    Level I Basic is required of all animal users, animal handlers and IACUC members, and it covers the rules and regulations regarding animal use at ASU and alternatives to animal use.  Recertification is required every four (4) years.  Level I training is an online training module.

    Level II Species is required of all animal users and animal handlers who come into direct contact with live animals. This training is species specific, and each user must be certified for each species of animal he or she will work with. Recertification is required every four (4) years. Level II training is an online training module.

    Level III covers training on specific procedures performed on animals and entails hands-on instruction by an individual (DACT or research personnel) with considerable experience with the procedure.  Evidence of training is required of all individuals who work with animals.  Procedures requiring Level III training include:

  • aseptic technique, anesthesia and post-surgical monitoring (must be provided by DACT veterinary staff)
  • surgery (can be provided by DACT staff or an experienced investigator)
  • blood collection (can be provided by DACT staff or an experienced investigator)
  • injections (can be provided by DACT staff or an experienced investigator)
  • oral dosing (can be provided by DACT staff or an experienced investigator)
  • euthanasia (can be provided by DACT staff or an experienced investigator)

Level III training should be deemed adequate once the individual has developed the skills to perform the procedure independently (without supervision).  Once this level of training is reached, the veterinarian providing the training, the individual’s principal investigator (PI), or an alternate contact designated by the PI in the protocol must submit a Level III Training Documentation Form to within five (5) business days.  

An individual cannot perform a procedure independently until a Level III Training Documentation Form has been submitted and approved by the IACUC.

ASU faculty members and medical professionals who have previous experience may substitute the hands-on training requirement by emailing the IACUC office a vita or a list of published work along with the Level III Training Documentation Form indicating those procedures they are skilled to perform under the protocol.  All other individuals, whether coming to ASU with experience or not, must receive training or have their skills verified by an experienced person prior to submitting the Level III Training Documentation Form.

DACT-provided training

DACT can provide the IACUC-required Level III training upon request and free of charge. This training can cover basic procedures including handling, delivery methods, sampling methods, anesthesia, aseptic technique and euthanasia on mice, rats, guinea pigs, rabbits, frogs, chickens and other species. In addition to being an IACUC requirement, appropriate training in these methods helps to ensure that animals are handled and treated humanely and that research results are consistent.  

Contact  to schedule a session. Records of all DACT-provided training will be sent to the IACUC to provide documentation of Level III compliance. Some training sessions are intended to enhance skills required for working in a particular area or with particular animals, and, therefore, some training sessions are by invitation only and may require multiple sessions. 

Safety training

Depending on the specifics of the research being performed, additional safety trainings may be required by Environmental Health & Safety (EH&S), including but not limited to:

  • Anesthetic Gas Safety – required when utilizing gas anesthesia
  • Hazardous Waste Management – required for discarding chemicals or biological materials
  • Laboratory Safety – required when working in a research laboratory
  • Nonhuman Primate Safety – required when working with nonhuman primates
  • Radiation Safety – required when using irradiation or radiological materials
  • Venomous Reptile – required when working with venomous reptiles

The EH&S Training Determination Tool can be used to help determine safety courses based on the work being performed.

Training resources

Additional, optional training on topics such as occupational health and safety and aseptic surgery can be found on the IACUC Training site.  

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